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. 2019 Jul 24;2(4):e201900421. doi: 10.26508/lsa.201900421

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© 2019 Banerjee et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S1. — (A) Progressive reduction of the TbPex3 transcript with time of RNAi induction. Semiquantitative RT-PCR analysis of transcript levels for TbPex3 and Tubulin (control) from uninduced BSF cells (−Tet) and RNAi-induced BSF cells (+Tet) at 4, 6, and 8 h of induction. (B) TbPex3-RNAi leads to increasing numbers of aberrant glycosome structures, glycosome fragmentation, and mislocalization of the glycosomal matrix enzyme aldolase to the cytosol with time of RNAi incubation. Uninduced BSF cells (−Tet) and TbPex3-RNAi–induced BSF cells induced for 4, 6, and 8 h were processed for immunofluorescence microscopy with rabbit anti-aldolase antibodies and Alexa Fluor 488 goat antirabbit IgG. Bar, 5 μm.