Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 25;9:10825. doi: 10.1038/s41598-019-47232-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cis inhibition mediated by Dll1 inhibits Jagged-induced differentiation. (a–c) Q-RT PCR analysis of mRNA levels of Notch ligands (a), Notch target genes (b) and terminal differentiation markers (c) in keratinocytes (strain km) transfected with two different Dll1-specific siRNAs or with a non-targeting control (NTC) siRNA and cultured under conditions that facilitated cell-cell interaction. Data shown are from n = 3 independent experiments performed with two biological replicates (independent siRNA transfections). Individual data points represent the average fold change in mRNA abundance (normalized to 18sRNA) compared to siNTC in each experiment. Bars represent the mean. P-values were calculated using one-way ANOVA with Holm Sidak’s multiple comparisons test (*p < 0.05). (d) Clonal growth of keratinocytes (strain km) transfected with the indicated siRNAs. Data shown are from n = 3 independent experiments performed with three technical replicates. Individual data points represent average percentage of colonies formed per number of cells seeded (colony formation efficiency, CFE); lines represent the mean. P-values were calculated using one-way ANOVA with Holm Sidak’s multiple comparisons test (*p < 0.05). (e) Schematic outline of the experimental setup used in (f,g). (f,g) Q-RT PCR analysis of terminal differentiation marker expression in keratinocytes (strain km, (f); strain kn, (g)) expressing zDLL1 or EV 48 hours after seeding onto substrates functionalized with the indicated proteins. Data shown in f and g are from n = 1 experiment performed with two technical replicates. Individual data points represent the average fold change in mRNA abundance (normalized to 18sRNA) compared to control (EV cells growing on substrates functionalized with anti-β2MG antibodies) in each experiment. Bars represent the means.