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. 2019 Jul 26;39(7):BSR20191221. doi: 10.1042/BSR20191221

Figure 4. CDK5 expression affects migration, invasion, proliferation, and EMT process in CC cells.

Figure 4

(A) CCK-8 assay demonstrated that CDK5 transfection enhanced cell proliferation in both Hela (B) and SiHa cell lines (*P<0.05, **P<0.01; by ANOVA vs control). (C) Wound-healing assay showed that CDK5 overexpression increases the migration ability of HeLa (D) and SiHa cells. Representative images were obtained at 40× magnification at the indicated hours (*P<0.05, **P<0.01; by Student’s t-test vs control). (E) The effect of CDK5 overexpression on cell invasion, as determined by Transwell assay, obtained at 100× magnification in HeLa (F) and SiHa cells (*P<0.05, **P<0.01; by Student’s t-test vs control). (G) Western blot analysis of E-cadherin, N-cadherin, and Vimentin expression in HeLa (H) and SiHa cells after transfection with pcDNA 3.1-CDK5, pcDNA 3.1, or in control group. β-actin was used as an internal control (*P<0.05, **P<0.01; by Student’s t-test vs control).