FIG 1.

Neutrophil-depleted mice have a higher bacterial burden despite similar limited bioavailability of amino acids in the lungs. (A to D) Mock-depleted (circles) and αGr1 (squares) mice were infected with 1 × 10³ CFU of WT K. pneumoniae or PBS (baseline) and sacrificed at 40 hpi. (A and B) BAL fluid was analyzed by NMR, and the percentage of each metabolite normalized to the total measured metabolites in the lung is reported. “^Tyr” is conditionally essential in the absence of Phe in mammals. (C) CFU/g lung for each mouse. (D) PMN population (Ly6G⁺ CD11b⁺) assessed by flow cytometry. Each symbol represents an individual mouse, and the bar represents the geometric mean. Each condition was tested with two to four mice per cohort in at least two independent experiments. The data are combined from all experiments. Statistical significance was determined using two-way ANOVA on log-transformed values with Bonferroni’s posttest (A and B) and the Student t test on log-transformed (C) and (D) nontransformed values. **, P < 0.01; ***, P < 0.001.