TABLE 1.

MICs and EOPs of sGR, aroA-encoded, and clinical K. pneumoniae strains

Straina	Genotype	Fold change in MICglyb	Mean EOP (%)c ± SEM on:
			250 μg/ml glyphosate		1,000 μg/ml glyphosate
			24 h	48 h	24 h	48 h	72 h
MKP220	WT	1	0	80 ± 13	0	H	103 ± 17
RJS196	sGR1	6	105 ± 18	105 ± 18	TSTC	71 ± 1	71 ± 1
RJS197	sGR2	10	119 ± 19	119 ± 19	TSTC	91 ± 15	91 ± 15
RJS198	sGR3	8	86 ± 20	86 ± 20	TSTC	65 ± 9	65 ± 9
RJS199	sGR4	8	94 ± 15	94 ± 15	TSTC	71 ± 7	71 ± 7
RJS200	sGR5	10	105 ± 14	105 ± 14	TSTC	77 ± 3	77 ± 3
RJS201	sGR6	4	80 ± 7	80 ± 7	TSTC	79 ± 8	79 ± 8
ALM121	aroAG96A	4d	0	89 ± 11	0	94 ± 12	94 ± 12
ALM122	aroAT97I	2	79 ± 4	79 ± 4	TSTC	64 ± 4	64 ± 4
ALM123	aroAP101S	2	86 ± 14	86 ± 14	TSTC	71 ± 5	71 ± 5
ALM111	aroATIPS	8	H	114 ± 33	H	107 ± 32	107 ± 32
R1	ESBL	2	ND	ND	ND	ND	ND
R9	ESBL	4	ND	ND	ND	ND	ND
R17	KPC	2.7	ND	ND	ND	ND	ND
B1	Non-ESBL/KPC	4	ND	ND	ND	ND	ND
B6	KPC	1.7	ND	ND	ND	ND	ND
B17	ESBL	2.7	ND	ND	ND	ND	ND
B18	KPC	5.3	ND	ND	ND	ND	ND
B19	Non-ESBL/KPC	3.3	ND	ND	ND	ND	ND
B20	ESBL	2	ND	ND	ND	ND	ND

^aR1, R9, etc., are respiratory isolates; B1, B6, etc., are blood isolates.

^bMIC_gly of WT = 125 μg/ml ± 12. Bacteria were grown overnight in minimal media and two-fold serial dilutions of glyphosate. Experiments were performed three independent times in technical triplicate, and values are reported as the average fold change for three experiments.

^cEOP, efficiency of plating measured in three independent experiments, as described in Materials and Methods. Technical replicates were averaged in each experiment, and the EOP was calculated as CFU_+glyphosate/CFU_M9+glucose. Values represent the average EOP and SEM, as calculated by ANOVA with Dunnett’s t test. TSTC, too small to count; H, hazy growth on concentrated spots where more than 1,000 colonies would be expected but no colonies detected on samples expected to generate 100 colonies. ND, not determined.

^daroA^G96A has a growth defect in minimal media; the MIC_gly was thus determined by evaluating growth in glyphosate at 24 h.