TABLE 2.
Putative target proteins of disulfiram identified in the genome-derived proteome of Pythium insidiosuma
| Target proteins of disulfiram | Accession no. of query protein sequence | BLASTP against P. insidiosum proteome |
TBLASTN against transcriptome (E value) | No. of mapped peptide(s) in mascot library | ||
|---|---|---|---|---|---|---|
| Matched protein ID | E value | Accession | ||||
| Dopamine beta-hydroxylase (DBH) | P09172 | No match | ||||
| Carbamate kinase (CK) | 4OLC | No match | ||||
| Urease (URE) | 4H9M | PINS00920015C | 0.00 | GAX98700.1 | 0.00 | 40 |
| Aldehyde dehydrogenase (ALDH2) | P05091 | PINS02440013A | 3E–120 | GAY03418.1 | 0.00 | No match |
| PINS00560032A | 4E–66 | GAX96905.1 | 0.00 | 13 | ||
| PINS00240041A | 7E–66 | GAX94676.1 | 5E–179 | No match | ||
| PINS00460028A | 7E–61 | GAX96345.1 | 0.00 | No match | ||
| PINS00450059A | 2E–51 | GAX96237.1 | 0.00 | 21 | ||
| PINS00090050A | 1E–43 | GAX93307.1 | 0.00 | No match | ||
| PINS00450060A | 4E–43 | GAX96236.1 | 0.00 | No match | ||
| PINS00530007C | 6E–30 | GAX96738.1 | 0.00 | No match | ||
| PINS01590003A | 1E–22 | GAY01314.1 | 0.00 | No match | ||
| PINS00850003A | 5E–22 | GAX98398.1 | 0.00 | 14 | ||
| PINS00030096A | 5E–09 | GAX92574.1 | 0.00 | 18 | ||
a
The query protein sequences are deposited in the PDB (i.e., CK and URE) or UniProt (i.e., DBH and ALDH2) database. The transcriptome and in-house mascot proteome of P. insidiosum were used to validate the matched proteins.