FIG 4.
In vitro selection of HIV-1variants resistant against GRL-057-14, GRL-058-14, GRL-059-14, ATV, and DRV. A mixture of 11 multi-PI-resistant viruses (HIV11MIX) was propagated in MT-4 cells in the presence of increasing concentrations of each compound. When exposed to ATV (closed circles), the virus became resistant to the drug by passage 10, and the virus propagated even in the presence of 10 μM ATV by the end of passage 12. The development of DRV-resistant viruses was delayed compared to that of ATV, but the virus propagated even in the presence of 5 μM DRV by the end of passage 23 (closed triangles). HIV11MIX exposed to GRL-057-14, GRL-058-14, and GRL-059-14 replicated only poorly around 0.05 μM and beyond, and drug-resistant variants did not emerge throughout the selection periods.