FIG 3.

Effect of T-2307 on oxygen consumption in isolated yeast mitochondria. Oxygen consumption of isolated mitochondria was measured using a MitoXpress probe. The inhibitory effects of T-2307, oligomycin A (OLI), and KCN were evaluated in the presence of ADP (ADP-stimulated condition) (a) and in the absence of ADP (basal condition) (b). Oxygen consumption rates (OCRs) were determined from the slope of the initial 7.5 min of the time-oxygen concentration curve. The values represent the mean and standard deviation values of pooled data from two independent experiments, each performed in triplicate (total six replicates per group). Statistical analysis was performed using the parametric Dunnett’s multiple-comparison test. Asterisks indicate a significant difference from the control (**, P < 0.01; ***, P < 0.001).