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. 2019 Oct;217:119284. doi: 10.1016/j.biomaterials.2019.119284

Fig. 4.

Fig. 4

Electron scanning microscopy and immunohistochemistry of graft explants. (A) Electron microscopy images showing the internal surface of the explanted acellular graft compared with the cellularized graft and the pulmonary artery adjacent to the graft (Scale bar = 100 μm). (B) Fluorescence microscopy images showing endothelial cells (isolectin, green) lining the internal lumen and surrounding layer of VSMCs (αSMA, red). Arrows indicate vessels (Scale bar = 90 μm). (C) SMA expression quantification and smooth muscle cells (SMC) layer thickness in acellular and cellularised grafts. Student's t-test was used. *p < 0.05. (D) Representative images of the immunofluorescent staining of CNN1 in explanted acellular and cellularized grafts (Scale bar = 50 μm). (E) Representative images of the immunofluorescent staining of SMHC in explanted acellular and cellularized grafts (Scale bar = 50 μm). The adjacent left pulmonary artery was used as a control sample.