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. 2019 Jul 19;10:1629. doi: 10.3389/fimmu.2019.01629

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Copyright © 2019 Mengrelis, Lau, Rowell, Solanki, Norris, Ross, Ono, Outram and Crompton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hh-mediated transcription in γδ T-cells influences γδ T-cell populations in adult spleen. (A–D) Gli2ΔN2-transgenic and WT littermate spleens were analyzed by flow cytometry. In scatter plots each point represents data from a different mouse; bars show mean ± SEM. (A) The number of CD3+γδ+ cells in the spleen. (B) Representative dot plot shows anti-CD27 and anti-CD44 staining gated on CD3+γδ+ cells from WT and Gli2ΔN2-tg spleen, giving the percentage of cells in the regions shown. Scatter plot shows the percentage of CD27-CD44+ cells, gated on CD3+γδ+ cells in the spleen. (C) Representative dot plot shows anti-CD200 and anti-CD73 staining, gated on CD3+γδ+CD25-CD371- cells from WT and Gli2ΔN2-tg spleen, giving the percentage of cells in the region shown. Scatter plots show the percentage of CD200-CD73- cells, gated on CD3+γδ+CD25-CD371- cells, and the number of CD200-CD73-CD3+γδ+CD25-CD371- cells in WT and Gli2ΔN2-tg spleen. (D) Representative histograms show anti-Vγ2 staining, gated on CD3+γδ+ cells in the spleen, giving the percentage of cells in the marker shown. Scatter plots shows the percentage of Vγ2+ cells, gated on CD3+γδ+ cells in the spleen. (E) Scatter plot shows the number of NK1.1+ cells, gated on CD3+γδ+ cells in the WT and Gli2ΔN2-tg spleen. ^*p < 0.05; ^**p < 0.01; ^***p < 0.005.