Figure 4.

Nuclear piston 3D cell migration. Human fibroblasts migrating within a confining 3D cell‐derived matrix switch to lobopodial migration, a migration mode in which the nucleus can serve as a piston. The nucleus is pulled forward by myosin II contractility via vimentin intermediate filaments that link to nesprin‐3 on the nucleus. This pulling forward of the nucleus pressurizes the anterior end of the cell to protrude a lobopodial process. New cell‐matrix adhesions then form at the cell anterior to anchor the cells, and the cycle can repeat with another round of nuclear piston movement [Colour figure can be viewed at wileyonlinelibrary.com]