Figure 7.

Self‐assembly of dissociated epithelial cells into bud‐like organoid structures. A, Isolated embryonic salivary gland epithelia were dissociated into single cells and then cultured within a small 3D Matrigel microenvironment on a nuclepore membrane filter. Phase‐contrast time‐lapse microscopy shows rapid self‐aggregation of the initially dissociated cells (B) into clusters that merge into large aggregates, from which bud‐like structures protrude (C) during a process of self‐organization [Colour figure can be viewed at wileyonlinelibrary.com]