Skip to main content
. 2019 Jul 19;10:1711. doi: 10.3389/fimmu.2019.01711

Figure 2.

Figure 2

LncATV promotes HCV infection. (A) Huh7 cells were transfected with negative control siRNA (siNC) or siRNAs targeting lncATV. Gene silencing effects were measured by qRT-PCR. (B) Huh7 cells were transfected with various siRNAs at 24 h prior to Jc1-Luciferase reporter HCVcc infection. Luciferase activity was assayed at different timepoints post-infection. (C–E) As described in (B) except for the reporter HCVcc replaced by JFH-1 HCVcc. Intracellular HCV RNA (C) and viral core protein (D) were quantified by qRT-PCR with specific primers and Western blotting with indicated antibodies. Intracellular HCV core expression was detected by immunofluorescent staining (E). Scale bars, 30 μm. (F–H) Overexpression of lncATV exerts anti-HCV effect. Huh7 cells were transfected with lncATV expression plasmid or empty vector (EV) as a negative control at 24 h prior to Jc1-Luciferase reporter HCVcc infection. At 72 h or indicated timepoints post-infection, levels of lncATV (F), luciferase activity (G), and HCV core protein (H) were measured by qRT-PCR and Western blotting. The data represented mean ± SD (n ≥ 3). **P < 0.01, ***P < 0.001.