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. 2019 Apr 1;28(15):2459–2476. doi: 10.1093/hmg/ddz062

Table 1.

List of primers used for LA-PCR analysis and their sequence

Gene target Primer pair
HPRT (10.4 kb) 5′-TGGGAT TACACGTGTGAACCAACC-3′
5′-GCTCTACCCTGTCCTCTACCGTCC-3′
Polβ (12.2 kb) 5′-CAT GTC ACC ACT GGA CTC TGC AC-3′
5′-CCT GGA GTA GGA ACA AAA ATT GCT G-3′
NANOG (8.6 kb) 5′-CTCCGGAATGGTAGTCTGAGAA GAA-3′
5′-ATTTAGGGCAGGCACAAGATGG-3′
OCT ¾ (10.1 kb) 5′-TCTGTGGCCTCACCCTATGA-3′
5′-CAGACCTGTGGCAGGTATTGAA-3′