Figure 6.

Aryl hydrocarbon receptor activation increased the level of active LCK. B cells were treated with VH (0.02% DMSO) or TCDD (0.1, 1, and 10 nM) and activated by soluble human recombinant CD40 ligand and cytokines (IL-2 and IL-21) for 7 days. (A) Ratio of inhibitory pLCK (Tyr505) versus total LCK and (B) ratio of active pLCK (Tyr505) versus inhibitory pLCK. The ratio was calculated based on the flow cytometry measurement of total LCK and pLCK (Tyr505). (C) Percent total ZAP70+ B cells measured by flow cytometry on day 7. Significant differences from VH control are indicated by **p<0.01 by 1-way ANOVA followed by Fisher’s LSD post hoc test. Results are presented as the normalized percentage compared with the VH group. “N” indicates the number of donors used in the study.