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. Author manuscript; available in PMC: 2019 Jul 26.
Published in final edited form as: Cell Cycle. 2010 May 15;9(9):1830–1838. doi: 10.4161/cc.9.9.11543

Figure 4-.

Figure 4-

Analysis of romidepsin-induced changes in gene expression. Panel A. Cell lines were incubated with IC80 concentration of romidepsin for various periods of time; total RNA was isolated and evaluated by Northern analysis for expression of N-myc and p21 mRNA. 18S RNA serves as a loading control. Panel B. Cell lines were incubated with IC80 concentration of romidepsin for various periods of time; protein was extracted and evaluated by Western analysis for expression of N-myc and p21. Blots were reprobed with actin as a loading control. Panel C. Effect of romidepsin on secreted VEGF in NB cell lines. NB cell lines were exposed to IC80 concentrations of romidepsin for 4, 8 and 24h, supernatant collected, centrifuged and analyzed by ELISA for VEGF.