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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Clin Cancer Res. 2019 Feb 4;25(10):2996–3005. doi: 10.1158/1078-0432.CCR-18-3309

Figure 1. Single-cell isolation and sequencing of peripheral blood and Sézary Syndrome cells.

Figure 1

(A) Schematic of the isolation, sequencing, and analysis of the single-cells. (B) Flow cytometry gating of the patient sample to isolate peripheral blood and tumor cells. (C) tSNE projection of patient sample with normal peripheral blood samples (n=4436) outline in grey and tumorigenic CD4 cells (n=3443) in orange. (D) Unique significant cluster genes without overlap between clusters and based on the Wilcoxon rank sum test, adjusted P-value < 1e-50. (E) Phylogenic tree of cluster identities based on mean mRNA values in the cluster with corresponding cluster proportion of cells composition. (F) Quantile-normalized Spearman correlation values of predicted immune cell phenotype based on SingleR algorithm for each tSNE cluster.