KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Myosin7a | Proteus Biosciences | Cat Num 25–6790; RRID:AB_2314839 |
| Myosin7a | DSHB | Cat Num MY07A 138–1; RRID:AB_2282417 |
| Sox2 | Santa Cruz Biotechnology | Cat Num sc-17320; RRID:AB_2286684 |
| AnnexinA4 | R&D Systems | Cat Num AF4146; RRID:AB_2242796 |
| Osteopontin | R&D Systems | Cat Num AF808; RRID:AB_2194992 |
| Tuj1 | Neuromics | Cat Num MO15013; RRID:AB_2737114 |
| Neurofilament | Abcam | Cat Num ab4680; RRID:AB_304560 |
| Ctbp2 | BD Transduction Laboratories | Cat Num 612044; RRID:AB_399431 |
| Shanki | Santa Cruz Biotechnology | Cat Num sc-23543; RRID:AB_2301736 |
| Ki67 | Abcam | Cat Num ab16667; RRID:AB_302459 |
| Atohl | Proteintech | Cat Num 21215-1-AP; RRID:AB_10733126 |
| HA | Sigma-Aldrich | Cat Num ab9110; RRID:AB_307019 |
| Alexa Fluor donkey anti-goat 488 | Thermo Fisher Scientific | Cat Num A11055; RRID:AB_2534102 |
| Alexa Fluor donkey anti-goat 546 | Thermo Fisher Scientific | Cat Num A11056; RRID:AB_142628 |
| Alexa Fluor donkey anti-goat 647 | Thermo Fisher Scientific | Cat Num A21447; RRID:AB_141844 |
| Alexa Fluor donkey anti-mouse 488 | Thermo Fisher Scientific | Cat Num A21202; RRID:AB_141607 |
| Alexa Fluor donkey anti-mouse 546 | Thermo Fisher Scientific | Cat Num A10036; RRID:AB_2534012 |
| Alexa Fluor donkey anti-mouse 647 | Thermo Fisher Scientific | Cat Num A31571; RRID:AB_162542 |
| Alexa Fluor donkey anti-rabbit 488 | Thermo Fisher Scientific | Cat Num A21206; RRID:AB_2535792 |
| Alexa Fluor donkey anti-rabbit 546 | Thermo Fisher Scientific | Cat Num A10040; RRID:AB_2534016 |
| Alexa Fluor donkey anti-rabbit 647 | Thermo Fisher Scientific | Cat Num A31573; RRID:AB_2536183 |
| Phalloidin | Thermo Fisher Scientific | Cat Num A22287; RRID:AB_2620155 |
| DAPI | Thermo Fisher Scientific | Cat Num D1306; RRID:AB_2629482 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 3,3’-iminodipropionitrile | Pfaltz & Bauer | #I00455 |
| Tamoxifen | Sigma-Aldrich | #T5648–1G |
| EdU | Thermo Fisher Scientific | #A10044 |
| NaOH | Thermo Fisher Scientific | S318–500 |
| Tris-HCI | Thermo Fisher Scientific | #15568025 |
| Ketamine | Vedco | #NDC 50989-161-06 |
| Xylazine | Santa Cruz Animal Health | #SC-36294Rx |
| Critical Commercial Assays | ||
| Alexa Fluor 647 EdU Detection Kit | Thermo Fisher Scientific | #C10340 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.Cg-Tg(Plp1-cre/ERT)3Pop/J | Jackson Laboratory | RRID:IMSR_JAX:005975 |
| Mouse: B6;129S6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | Jackson Laboratory | RRID:IMSR_JAX:007908 |
| Mouse: CAG-flox-Atoh1-HA | Liu et al., 2012 | n/a |
| Mouse: Crl:CD1(ICR) | Charles River | RRID:IMSR_CRL:22 |
| Oligonucleotides | ||
| 5’-ACGCTCTGTCGGAGTTGCTG-3’ | n/a | Atohl-HA transgene (forward) |
| 5’-AGGGATAGCCCGCATAGTCA-3’ | n/a | Atohl-HA transgene (reverse) |
| 5’-CTAGGCCACAGAATTGAAAGATCT-3’ | n/a | Plp1-Cre internal positive control (forward) |
| 5’-GTAGGTGGAAATTCTAGCATCC-3’ | n/a | Plp1-Cre internal positive control (reverse) |
| 5’-GCGGTCTGCCAGTAAAAACTATC-3’ | n/a | Plp1-Cre transgene (forward) |
| 5’-GTGAAACAGCATTGCTGTCACTT-3’ | n/a | Plp1-Cre transgene (reverse) |
| 5’-CTGTTCCTGTACGGCATGG-3’ | n/a | tdTomato mutant (forward) |
| 5’-GGCATTAAAGCAGCGTATCC-3’ | n/a | tdTomato mutant (reverse) |
| 5’-AAGGGAGCTGCAGTGGAGTA-3’ | n/a | tdTomato wild type (forward) |
| 5’-CCGAAAATCTGTGGGAAGTC-3’ | n/a | tdTomato wild type (reverse) |
| Software and Algorithms | ||
| Fiji V2.0.0 | NIH | n/a |
| GraphPad Prism v7.0c | GraphPad | n/a |
| RStudio v0.99.891 | RStudio, Inc. | n/a |
| Other | ||
| PBS | Sigma-Aldrich | #T5648–1G |
| BSA | Thermo Fisher Scientific | #BP1600–100 |
| NaN3 | Sigma-Aldrich | #S2002–25G |
| DAKO | Agilent | #S3023 |