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. 2019 Jul 19;12:178. doi: 10.3389/fnmol.2019.00178

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Copyright © 2019 Wang, La and Hamill.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression index (ε) estimated for different genes in larger versus smaller DRG neurons. Left, Gene expression parameters estimated in analysis of 257 larger neurons (≥600 μm²). (A) Histograms showing the percentage of neurons expressing PIEZO1, PIEZO2, TRPV1, and PPIB genes. (B) Histograms showing the measured average gene transcript density (transcripts/μm²) in neurons that expressed the specific gene. (C) Histograms of the expression index calculated as the product of the % of neurons expressing the gene and their average transcript density (transcripts/μm²). Right, Expression parameters for 354 smaller neurons (≤500 μm²). (D) Histograms showing the percentage of neurons expressing PIEZO1, PIEZO2, TRPV1, and PPIB genes. (E) Histograms showing their average transcript density (transcripts/μm²) in neurons that expressed the specific gene. (F) Histogram of the expression index calculated as the product of the percentage of neurons expressing the gene and their average gene density transcripts/μm².