Figure 2.

BAY1143572 and 5-fluorouracil synergistically inhibit esophageal adenocarcinoma in vitro and in murine xenografts. (A) Cells pretreated with 5-fluorouracil (5 µM for FLO-1 cells, 10 µM for OE33 and SKGT4 cells) for 24 h were treated with BAY1143572 at the indicated doses for 48 h and then analyzed for cell proliferation by MTS assay. Data are the means ± standard error (SE) of three independent experiments. (B) Cells treated with BAY1143572 with or without 5-fluorouracil were stained with Annexin V–FITC and propidium iodide. Apoptosis was determined by flow cytometry. Data are the means ± SE of 3 independent experiments. ^*p < 0.05. (C) and (D) The xenograft-bearing mice were treated with BAY1143572 (12.5 or 15 mg/kg for FLO-1 xenografts, 15 mg/kg for ESO-26 xenografts) daily for 10 days and/or 20 mg/kg 5-fluorouracil every 3 days for 2 weeks by intraperitoneal injection. Tumor growth was measured as tumor volume. Data are the percentages of tumor growth.

^*p < 0.05.