Figure 5.

Effects of BAY1143572 plus 5-flououracil on MCL-1 protein and RNA levels in in vitro models of esophageal adenocarcinoma. (A) Lysates from cells treated with 1 µM BAY1143572 with or without 5-fluorouracil (10 µM for SKGT4 cells, 5 µM for FLO-1 cells) for 4 h were subjected to Western blotting for MCL-1. (B) Cells were treated with 1 µM BAY1143572 with or without 5-fluorouracil (10 µM for OE33 and SKGT4 cells, 5 µM for FLO-1 cells) for 4 h, and their MCL-1 mRNA levels were measured by quantitative real-time polymerase chain reaction (qPCR). Data are the means ± standard error (SE) of three independent experiments. (C) Western blot of esophageal adenocarcinoma cell lines with stable overexpression of MCL–1. (D) Cells with or without MCL-1 overexpression were treated with 5-fluorouracil and/or BAY1143572 at the indicated doses and then stained with Annexin V–FITC and propidium iodide. Apoptosis was analyzed by flow cytometry. Data are the means ± SE of three independent experiments.

^*p < 0.05 compared with control cells.