Table 2.
Infection, disseminated infection and transmission rates of Ae. caspius from Catalonia and Ae. aegypti PAEA populations artificially fed with Suriname and MR766 ZIKV strains at a titer of 7 log10 TCID50/ml. Viral detection was performed by the visualization of cytopathic effect on cells and RT-qPCR assays
| Dpe | Species | Suriname | MR766 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IR (%) | DIR (%) | TR (%) | IR (%) | DIR (%) | TR (%) | ||||||||
| CE | RT-qPCR | CE | RT-qPCR | CE | RT-qPCR | CE | RT-qPCR | CE | RT-qPCR | CE | RT-qPCR | ||
| 7 | Ae. caspius | 0/30 (0) | 0/30 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/30 (0) | 1/30 (3.33) | 0/0 (0) | 0/1 (0) | 0/0 (0) | 0/0 (0) |
| Ae. aegypti | 17/20 (85) | 20/20 (100) | 0/17 (0) | 0/20 (0) | 0/0 (0) | 0/0 (0) | 2/20 (10) | 2/20 (10) | 0/2 (0) | 0/2 (0) | 0/0 (0) | 0/0 (0) | |
| 14 | Ae. caspius | 0/30 (0) | 1/30 (3.33) | 0/0 (0) | 0/1 (0) | 0/0 (0) | 0/0 (0) | 0/30 (0) | 0/30 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) |
| Ae. aegypti | 20/20 (100) | 20/20 (100) | 9/20 (45) | 19/20 (95) | 3/9 (33.3) | 8/19 (52.6) | 2/20 (10) | 2/20 (10) | 0/2 (0) | 0/2 (0) | 0/0 (0) | 0/0 (0) | |
| 21 | Ae. caspius | 0/30 (0) | 3/30 (10) | 0/0 (0) | 0/3 (0) | 0/0 (0) | 0/0 (0) | 0/30 (0) | 0/30 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) | 0/0 (0) |
| Ae. aegypti | 19/20 (95) | 19/20 (95) | 16/19 (84.2) | 19/19 (100) | 1/16 (6.2) | 17/19 (89.4) | 1/19 (5.2) | 1/19 (5.2) | 0/1 (0) | 0/1 (0) | 0/0 (0) | 0/0 (0) | |
Abbreviations: CE, cytopathic effect; IR, infection rate; DIR, disseminated infection rate; TR, transmission rate; dpe, days post-exposure
Note: Data are given as number of positive/number of tested samples (percentage)