Fig. 2.

SAE1 knockdown decreases glioma cell proliferation and migration. a The interference effects of three specific SAE1 siRNAs in U87 and U251 cells. The siRNA-3 against SAE1 had the most effective gene inhibition. b SAE1 siRNA (siSAE1–3) decreases U87 and U251 cells proliferation. Cell proliferation was detected at transfection for 0, 12, 24, 36, 48, 60 h in glioma cells. Data are represented as the mean ± SD of three separate experiments. *p < 0.05. c The transwell assay was used to detect cell migration ability. Cells were observed at 24 h after transfection with 100 nM siSAE1–3 in U87 and U251 cells. d Cell migration was measured with wound healing assay after transfection for 24, 48 h. And cell migration distances were calculated relative to the initial distance before migration. siCon: non-targeting control siRNA. siSAE1: The SAE1-specific siRNA-3 (siSAE1–3). Data were presented as mean ± SD of three separate experiments. *p < 0.05, **p < 0.01