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. 2019 Jul 26;52:38. doi: 10.1186/s40659-019-0245-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — miR-29b-3p inhibitor induces apoptosis. A TUNEL assay. The nuclei were visualized by staining with DAPI (blue), Cy3-labeled the apoptosis cells (red). Image magnification: ×20. B Annexin V-FITC and propidium iodide assay. The sum of early and late apoptotic cells ratio (%) were quantitated by flow cytometer analysis of Annexin V/PI. C Hoechst staining. (a) MDA-MB-231; (b) MCF-7. White arrows indicate the cells nuclear fragmentation. The nuclei were visualized by staining with Hoechst (red). Image magnification: ×40. D Western blot for Cleaved PARP. Data are presented as the mean ± SD of three independent experiments. The data represents three experiments exhibiting similar results