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. 2019 Jul 25;15:81. doi: 10.1186/s13007-019-0467-z

Fig. 5.

Fig. 5

Representative MRM chromatograms of SW5 samples, non-spiked and spiked with a mixture of KAR1 (a) and KAR2 (b) at two concentrations (0.5 and 1 μmol/l). Reproducibility is shown in triplicates (1st injection blue, second injection red, third injection black). In the boxes, representative calibration curves constructed from five SW matrix-based solutions spiked with a mixture of KAR1 and KAR2 at known concentrations (0, 0.25, 0.5, 0.75 and 1.0 μmol/l). The samples were analysed by UHPLC-ESI(+)-MS/MS using the standard addition method