Table 1.
Partition coefficients, retention times, diagnostic MRM transitions and optimised instrument settings for the synthesised and studied karrikins
| Compounds | log P | Rt (min) | CV (V) | Quantitation MRM transitions | CE (eV) | Confirmation MRM transitions | CE (eV) | LOD (fmol) |
|---|---|---|---|---|---|---|---|---|
| KAR1 | − 0.72 ± 0.46 | 3.62 ± 0.02 | 35 | 151 > 123 | 18 | 151 > 67 | 21 | 1.0 |
| KAR2 | − 1.31 ± 0.46 | 2.09 ± 0.01 | 30 | 137 > 81 | 19 | 137 > 109 | 16 | 0.1 |
| KAR-BR | − 0.96 ± 0.50 | 3.81 ± 0.02 | 25 | 215 > 136 | 22 | 215 > 80 | 27 | – |
Rt retention time, CV cone voltage, CE collision energy, LOD limit of detection