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. 2019 Jul 19;10:818. doi: 10.3389/fphar.2019.00818

Figure 1.

Figure 1

Characterization of carmofur as a fatty acid amide hydrolase (FAAH) and N-acylethanolamine acid amidase (NAAA) dual inhibitor. Concentration-dependent inhibition of HEK293-rFAAH (A) and HEK293-rNAAA (B) by carmofur. Effects of dialysis (0, 6 h; 0–4°C) on the activities of FAAH (C) and NAAA (D) by carmofur (5 µM). Results are expressed as mean ± SEM, n = 3 replicates per group. ***P < 0.001 vs. vehicle control by one-way ANOVA. Effect of vehicle (1% DMSO) or 5-fluorouracil (20 µM) on FAAH (E) and NAAA (F) activities in HEK293 cells heterogeneously overexpressing FAAH and NAAA. Competition binding assay of vehicle (1% DMSO), GW7647 (10 µM), (R)-(+)-WIN 55212-2 (5 µM), and carmofur (20 µM) for PPARα (G) and CB2 (H).