Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 19;9:640. doi: 10.3389/fonc.2019.00640

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Patel, Norman, Chang, Abedi, Liu, Chwa, Atilano, Thaker, Lu, Jazwinski, Miceli, Udar, Bota and Kenney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) The J cybrids were more sensitive to cisplatin treatment than the H cybrid cultures. Cell viabilities of the untreated and cisplatin-treated cybrids were measured using the trypan blue dye exclusion assay and normalized to the untreated-H cybrid sample (Cyb H Unt, 100.0 ± 16.3). The viability in untreated-J cybrids increased at 2.26-fold higher rate than the untreated-H cybrids (P = 0.001). The viability declined in the 25 μM cisplatin-treated-H cybrids (13%, P = 0.58) and 50 μM cisplatin-treated-H cybrids (38%, P = 0.05) compared to the untreated-H cybrids. The viability decreased in the 25 μM cisplatin-treated-J cybrids (35%, P = 0.05) and 50 μM cisplatin-treated-J cybrids (65%, P = 0.002) compared to the untreated-J cybrids. Each experiment was repeated twice and analyzed in triplicate. (B) J cybrids have a greater loss of mitochondrial membrane potential (ΔΨm) after cisplatin treatment than H cybrids. The ΔΨm values in cisplatin-treated-J cybrids were significantly decreased compared to the untreated-J cybrids (P = 0.0001) and also the cisplatin-treated H cybrids (P = 0.0005). In contrast, the cisplatin-treated and untreated-H cybrids showed similar levels to each other (P = 0.51). ΔΨm, mitochondria membrane potential; RFU, relative fluorescent units. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. (C) Cisplatin-treated-H cybrids produced higher levels of ROS compared to the cisplatin-treated-J cybrids. The ROS levels, as measured in relative percentile, were compared between the H and J cybrids without and with cisplatin treatments after 48 h. The cisplatin-treated-J cybrids had significantly lower ROS levels compared to the untreated-J cybrids (P = 0.03) and also compared to the cisplatin-treated-H cybrids (P = 0.006). The ROS levels for the untreated H and cisplatin-treated H were similar to each other (P = 0.37). RFU = relative fluorescent units. ^*P < 0.05.