Table 2A.
Description of genes analyzed in cisplatin treated H and J cybrids.
| Symbol | Gene name | Genbank accession numbers | Functions |
|---|---|---|---|
| CANCER-RELATED | |||
| ABCC1 | ATP-Binding Cassette, SubFamily C |
NM_004996 | Known as MRP1 (multidrug resistance protein 1). Member of the ATP binding cassette family that transports molecules across membranes. Mutations in ABCC1 N-glycosylation connected with Cisplatin resistance. |
| ALK1 | Type 1 Cell-Surface Receptor for TGF-beta ligand superfamily | NM_000020 | Type Cell-Surface receptor for TGF-beta superfamily of ligands. Shares similar domain structures in serine-threonine kinase subdomains with other activing receptor-like kinase proteins. |
| BRCA1 | DNA Repair associated | NM_007294 | Nuclear Phosphoprotein that acts as a tumor suppressor by maintaining genomic stability. Involved in transcription, DNA repair of double-stranded breaks, and recombination. |
| BMI1 | Eukemia viral BMI-1 oncogene | NM_005180 | Stem cell marker. Has key role in regulating the proliferative activity of both normal and leukemic stem cells. |
| CDK2 | Cyclin dependent kinase 2 | NM_001798 | Associated with apoptosis. Essential for completion of prophase I during meiotic cell division in male and female germ cells. |
| CDKN1A/P21 | Cyclin-dependent kinase inhibitor 1A/ p21 |
NM_000389 NM_001220777 NM_001220778 NM_078467 NM_001291549 |
Plays a critical role in the cellular response to DNA damage and cisplatin toxicity. Mediates cell cycle arrest. Is cyto-protective. |
| CYP51A | Cytochrome P450, Family 51, Subfamily A, Polypeptide 1 |
NM_000786 NM_001146152 |
Member of the cytochrome P450 enzyme family of monooxygenases. |
|
DHRS2/ HEP27 |
Dehydrogenase/ Reductase (SDR Family) Member 2 |
NM_182908 NM_005794 |
NADPH-dependent dicarbonyl reductase activity. Mitochondrial matrix protein. Inhibits MDM2 and stabilizes p53. |
| EGFR | Epidermal Growth Factor Receptor | NM_005228 | Triggers cell proliferation when bound to epidermal growth factor |
| ERBB2 | Erb-b2 Receptor Tyrosine Kinase 2 | NM_004448 | Member of epidermal growth factor receptor family of receptor tyrosine kinases. Stabilizes binding of epidermal growth factor to receptor |
| ERCC1 | Excision Repair Cross-Complementation Group 1 |
NM_001166049 NM_001983 NM_202001 |
Nucleotide Excision Repair formed by electrophilic compounds such as Cisplatin. Forms a heterodimer with XPF endonuclease. Involved in recombination DNA repair, inter-stand crosslink, and lesion repair. |
|
KAT5/ TIP60 |
K (Lysine) acetyltransferase 5 |
NM_001206833 NM_006388 NM_182709 NM_182710 |
Drug resistance; has role in acetylation of histones; modulates DNA damage response. |
| SFRP1 | Secreted frizzled-related protein 1 | NM_003012 | Soluble modulators of Wnt signaling. Regulates cell growth and differentiation. Silencing leads to deregulated pathway, associated with cancer. |
| TP53 | Tumor protein p53 |
NM_000546 NM_001126112 NM_001126113 NM_001126114 NM_001126115 NM_001126116 NM_001126117 NM_001126118 NM_001276695 NM_001276696 NM_001276697 NM_001276698 NM_001276699 NM_001276760 NM_001276761 |
Regulates genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Induces apoptosis. |
| APOPTOSIS | |||
| BAX | BCL2-Associated X Protein |
NM_001291429 NM_001291428 NM_001291430 NM_138761 NM_004324 NM_138764 NM_001291431 NM_138763 |
Associates to form a heterodimer with BCL2. Functions in apoptotic behavior by opening the mitochondrial voltage dependent anion channel, leading to loss of membrane potential, and opening of cytochrome C. |
| BBC3 | BCL2 Binding Component 3 |
NM_001127240 NM_001127241 NM_014417 |
Member of the BCL-2 family. Induces mitochondrial membrane permeablization and apoptosis. |
| BCL2L13 | BCL2-Like-1 |
NM_015367 NM_001270729 NM_001270731 NM_001270732 NM_001270734 NM_001270735 |
BCL2 isoform. Mitochondrial-localized protein. Overexpression results in apoptosis. |
| CASP3 | Caspase-3 |
NM_004346 NM_032991 |
Effector caspase; Activated by caspases 8, 9, and 10. Effects caspases 6, 7, 9. Belongs to family of proteases involved in apoptosis; Synthesized as inactive precursors and therefore need activation. |
| CASP9 | Caspase-9 |
NM_001229 NM_032996 |
Part of the apoptosome protein complex formed during apoptosis. Mitochondrial caspase activation. |
| SIGNALING | |||
| EFEMP1 | EGF-Containing Fibulin-Like Extracellular Matrix Protein-1 |
NM_001039349 NM_001039348 |
Fibulin family of extracellular matrix glycoproteins. Binds to epidermal growth factor receptors causing phosphorylation and signaling. Associated with drug resistance and cancer prognosis. |
| FOXM1 | Forkhead Box M1 |
NM_001243088 NM_001243089 NM_021953 NM_202002 NM_202003 |
Transcriptional activator involved in cell proliferation. Regulates expression of several cyclins. Role in DNA break repairs. Phosphorylated and inactivated during mitosis. |
| MAPK8 | Mitogen-activated protein kinase 8 |
NM_002750 NM_139046 NM_139047 NM_139049 NM_001278547 NM_001278548 |
Targets specific transcription factors. Mediates immediate-early gene expression. Involved in UV radiation induced apoptosis. |
| MAPK10 | Mitogen-activated protein kinase 10 |
NM_002753 NM_138980 NM_138982 |
Regulatory role in signaling pathways during neuronal apoptosis; Inhibited by cyclin dependent kinase 5. |
| HOUSEKEEPERS | |||
| ALASV1 | 5'-amino-levulinate synthase 1 | NM_000688 | Mitochondrial enzyme catalyzes rate-limiting step in heme biosynthesis. Level of the mature encoded protein regulated by heme. |
| GUSB | Glucuronidase, beta |
NM_000181 NM_001284290 NM_001293104 NM_001293105 |
Belongs to the glycosidase family of enzymes that break down complex carbohydrates. |
| HMBS | Hydroxy-methylbilane synthase |
NM_000190 NM_001024382 NM_001258208 NM_001258209 |
Third enzyme of heme biosynthetic pathway; Catalyzes condensation of four porphobilinogen molecules into hydroxymethylbilane. |
| HPRT1 | Hypoxanthine phosphor-ribosyl-transferase 1 | NM_000194 | Transferase catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate. |
| TUBB | Tubulin beta class I |
NM_178014 NM_001293213 |
Part of protein superfamily of globular proteins. Major component of microtubules. |