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. 2019 Jul 19;10:1642. doi: 10.3389/fmicb.2019.01642

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Copyright © 2019 Osorio, Mettert, Kiley, Dopson, Jedlicki and Holmes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design and results of FNR_AF activity from the Escherichia coli promoter PnarG. (A) Experimental design to test the effect of FNR_AF (encoded on plasmid pKK223-3 fnr_AF) on in vivo expression of PnarG in E. coli Δfnr. (B) Data are reported as β-galactosidase activities (n = 3 ± SD, one way Anova p < 0.05) in strains RZ8480 A. f. [E. coli fnr (−) fnr A,f. (+)] in plasmid pKK223-3, PK7350 [E. coli fnr (+)], RZ8480 [E. coli fnr (−)], and RZ8480 PKK [E. coli fnr (–)] with pKK223-3 plasmid. The dotted arrow indicates the expression of FNR_AF from fnr_AF, its dimerization and binding to PnarG.