TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype | References or sources |
| At. ferrooxidans | ||
| ATCC 23270 | Wild type | |
| Escherichia coli | ||
| JM109 | endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ(lac-proAB), [F′ traD36, proAB, laqIqZΔM15] | Promega |
| BL21 | F-, ompT, hsdSB (rB-mB-), gal, dcm, rne131, (DE3) | Invitrogen |
| PK22 | hsdS, gaL λDE53, Δcrp-bs990, rpsL, Δfnr, zcj-3061::Tn10 | Lazazzera et al., 1993 |
| RZ7350 | lacZ Δ145, narG234::MudI1734 | Kiley and Reznikoff, 1991 |
| RZ8480 | Δfnr, lacZ Δ145, narG234::MudI1734 | Lazazzera et al., 1993 |
| Plasmids | ||
| pET100/D-TOPO | Expression vector | Invitrogen |
| pET100/D-TOPO FNRAF | Expression vector, FNRAF protein | This study |
| pKK223-3 | Plasmid vector, Ampr | PL-Pharmacia |
| pKK223-3 fnrAF | fnrAF coding region cloned into pKK223-3 vector with a tac promoter | This study |
| pET11A | Expresion vector, Ampr with a T7 promoter | Novagen |
| pET11A fnrAF | fnrAF coding region cloned into pET11A vector with a T7 promoter | This study |