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. 2019 Jul 26;9:10894. doi: 10.1038/s41598-019-46945-8

Figure 2.

Figure 2

Nested PCR results of anatoxin-a synthetase C (anaC) gene of a subset of cyanobacterial bloom samples separated by 1.5% agarose gel electrophoresis. DNA fragments at 366 bp indicate the anaC gene. The positive control represents a known ATX-a producing Cuspidothrix issatschenkoi CY1941. A known negative ATX-a producer, Nostoc punctiforme PCC73102 and non-template control (water), were used as negative controls. The ladder represents DNA fragments with 100 bp. For sample descriptions and sequencing results, see Supplementary Table S1. The present image is cropped and a full-length gel is presented in Supplementary Fig. S1.