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. 2019 Jul 26;9:10868. doi: 10.1038/s41598-019-47386-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tom1 VHS calorimetric titration curves for the association to PtdIns5P (a), Ins(1,5)P₂ (b), PtdCho (c), and ubiquitin (d). The upper panels depict the heat change produced upon successive injections of the indicated ligand to the Tom1 VHS domain. The lower panels display the integrated binding isotherms as a function of ligand/protein ratio. Binding isotherms were fitted to the subtracted data using two-sites (PtdIns5P) or one-site (Ins(1,5)P₂ and ubiquitin) binding models. Each titration was carried out at least two times and representative results are shown.