Figure 4.
Exopolysaccharides accumulation in the DEA613-adapted strain T56-1. (A) Differential expression of genes involved in colanic acid and cellulose biosynthesis. Log2FC values represent adapted T56-1 versus wt both grown under DEA613 (blue), and UPA230-grown wt versus SLA-grown wt (purple), respectively. (B) Colanic acid accumulation in both adapted strain T56-1 and wt grown under SLA or DEA613. To measure colanic acid, concentration of fucose, which is specific to the colanic acid monomer, was evaluated in the exopolysaccharide fraction94. (C) Cellulose accumulation in both adapted strain T56-1 and wt grown under SLA or DEA613. Cellulose in the exopolysaccharide fraction was digested with cellulase and the resulting glucose was measured96. Colanic acid and cellulose results were normalized with OD 600 nm and are the mean and standard deviation of three replicates. Fuc: fucose, Glu: glucose, Gal: galactose, Glc: glucuronic acid.