Fig. 2. Functional studies of eIF4E-associated lncRNAs.

a Bar graphs showing the effect of lncRNAs SNHG5, SNHG12, FTX, and ZNFX1 on growth of cells. The specified lncRNAs were knocked down by transfection of targeted siRNA, and 48 h after transfection cells were plated in 96-well plates for growth assessment by MTT assay. Bars represent mean ± SD from eight replicates; **p < 0.001. b Images of agarose gels showing total levels (left panel) and eIF4E-bound (right panel) lncRNAs SNHG12 and FTX in 293T cells transfected with empty vector (V), HA-eIF4E^WT, HA-eIF4E^Del1&2, or HA-eIF4E^M-cap lncRNA levels were detected by RT-PCR using total RNA (left) or RNA-IP with anti-HA antibody. β-Actin was used as a loading control. c Images of western blots showing protein levels of c-MYC, BCL-2, and BCL-XL in cells transfected with a control or lncRNA targeted siRNA to individually knock down each lncRNA