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. 2019 Jul 26;9:10882. doi: 10.1038/s41598-019-47244-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NDV related kinetics and cytotoxicity in SAS cells. The images show cell control, and NDV specific cytopathic effects (A). The images were taken at 20X magnification under EVOS FLoid cell imaging station (ThermoFisher Scientific, USA). SAS cells were infected with different MOI and MTT assay was performed at regular time intervals (B). Time-course viral growth kinetics were NDV was infected at different MOI and the supernatant was collected for virus titration by HA (C) and TCID₅₀ method (D). MTT, HA, and TCID₅₀ data represent the mean ± SD of three independent experiments.