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. 2019 Jul 26;10:3353. doi: 10.1038/s41467-019-11282-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Setd2-deficient mice exhibit reduced numbers of T and B lymphocytes. a Relative mRNA expression of Setd2 in different hematopoietic cell compartments (n = 4). b Schematic of the strategy for establishing the Setd2^f/f mouse line. c Immunoblotting of Setd2 and H3K36me3 in bone marrow nucleated cells (BMNCs) from Setd2 knockout mice. H3 and β-actin were used as the loading controls. d Immunohistochemistry of H3K36me3 in femur sections from Setd2 knockout and control mice. e Complete blood count of peripheral blood showed lymphopenia in pIpC-treated Mx1-Cre⁺;Setd2^f/f mice. (WBC, white blood cell; PBL, peripheral blood lymphocyte; n = 7). f, g Flow cytometric analysis of CD3e⁺ T cells (f) and B220⁺ B cells (g) in the peripheral blood of Setd2 knockout and control mice (n = 8–9). h–j Flow cytometric analysis of bone marrow and bone marrow lymphocytes (BMLYs) in Setd2 knockout and control mice. (n = 6). (Data were collected at 8 weeks after the final pIpC injection. The data are presented as the means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant, Source data are provided as a Source Data file)