Fig. 2.

Characterization of H2A.Z in wild type and mutant chromatin. a Western blot and quantification for H2A.Z and H3 levels in total extracts from wild type, mbd9-3, arp6-1, and arp6-1 mbd9-3. The upper band in the H2A.Z blot likely represents a modified form of H2A.Z. Only the lower band was used in the quantification. Average levels of three independent westerns ± standard deviations are shown. Paired two-tailed Student’s t-test was used to determine significance between wild type and mutants (black asterisks) or between mutants (red asterisks); ns p value >0.05, *p value ≤ 0.05, **p value ≤ 0.01, ***p value ≤ = 0.001. b Distribution of normalized H2A.Z ChIP-seq signal as read count kbp⁻¹ million⁻¹ mapped reads (RPKM) from merged replicates over H2A.Z common peaks in wild type and protein-coding genes. c Overlap of H2A.Z-depleted genes for mbd9-3, arp6-1, and arp6-1 mbd9-3 mutants. d–g Distribution of normalized H2A.Z ChIP-seq signal (RPKM) from merged replicates over H2A.Z-enriched genes for indicated mutants. h Intersection of H2A.Z-depleted genes from d to g with nine classes of genes characterized by their distribution of H2A.Z in wild type at TSS and gene body. Labels H—high, M—medium, L—low refer to levels of H2A.Z at TSS (first letter) and gene body (second letter). Source data for Figs. 2a and 2c are provided as Source Data file