Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 26;9:10864. doi: 10.1038/s41598-019-47391-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunopotency assay (IPA) with human activated PBMC and IFP-MSC in co-culture. The percentage of CFSE-labelled PMA/IO-activated PBMC proliferation (relative to unstimulated PBMC) was measured in PBMC only and in co-cultures with naïve and TI-primed IFP-MSC at various ratios (2:1, 4:1, 12:1, 60:1 PBMC:IFP-MSC). Naïve IFP-MSC (blue) abrogated PBMC proliferation in a declining dose-dependent manner, while TI-primed cells (red) sustainably affected PBMC proliferation even at a 12:1 ratio. At a 60:1 ratio, the interaction with either IFP-MSC slightly augmented PBMC proliferation. PBMC proliferation is quantified (top), whereas corresponding representative flow cytometry overlay data (bottom) shows proliferation magnitude with dotted line shape and arrow sizes. All co-cultures were performed in duplicate, with every MSC donor (n = 3) co-cultured in independent experiments with two different PBMC populations obtained from unrelated donors. Data are presented as scatter plots with mean ± SD, with 6 data points per conditions (3 MSC ×2 PBMC). Paired and unpaired t tests and one-way ANOVA for multiple comparisons were used for statistical analysis *p < 0.05.