Table 3.
KEGG pathway analysis for differentially methylated genes. We used missMethyl tool for pathway analysis. For each enriched pathways, N is the total gene in given pathways, DN is the number of mapped genes in hg38 against differentially methylated CpGs, P.DM is the p-value, and FDR is the BH adjusted P-value.
| Pathway | N | DM | P.DM | FDR |
|---|---|---|---|---|
| Neuroactive ligand-receptor interaction | 252 | 90 | 1.37E-09 | 4.51E-07 |
| Calcium signaling pathway | 173 | 73 | 5.36E-07 | 8.84E-05 |
| Rap1 signaling pathway | 203 | 77 | 3.40E-05 | 0.00374 |
| Nicotine addiction | 36 | 20 | 0.00014 | 0.01117 |
| MAPK signaling pathway | 283 | 96 | 0.00031 | 0.02041 |
| cAMP signaling pathway | 191 | 66 | 0.00037 | 0.02043 |
| Salivary secretion | 81 | 31 | 0.00064 | 0.03027 |
| Circadian entrainment | 95 | 40 | 0.00102 | 0.03834 |
| Morphine addiction | 88 | 38 | 0.00105 | 0.03834 |
| Mucin type O-glycan biosynthesis | 29 | 14 | 0.00132 | 0.04339 |