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. 2019 Jul 22;12:331–336. doi: 10.2147/CEG.S214196

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© 2019 Tsugawa et al.

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ERα is involved in the TNFα and IL-6 production after liver resection. Wild-type (WT) and knockout (KO) male mice underwent partial hepatectomy (PH or were sham-operated. (A) Circulating TNFα concentrations were analyzed by enzyme-linked immunosorbent assay at indicated times after PH. (B and C) ERα is involved in NF-κB activation after PH. NF-κB DNA-binding activity was analyzed with an electrophoretic mobility shift assay using liver extracts. NF-κB activity was confirmed by competition with other NF-κB binding sites (H2K and KF-κB, [B]). (D) Circulating IL-6 concentrations analyzed by enzyme-linked immunosorbent assay at indicated times after PH. (E and F) ERα is involved in STAT3 activation after PH. STAT3 DNA-binding activity was analyzed via an electrophoretic mobility shift assay using liver extracts. STAT3 activity was confirmed by competition with other STAT3 binding sites (E).