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. Author manuscript; available in PMC: 2019 Jul 26.
Published in final edited form as: ACS Catal. 2019 Apr 11;9(6):4764–4776. doi: 10.1021/acscatal.9b00231

Figure 1.

Figure 1

HPLC profile of the LmbB2 reaction shows L-tyrosine (1) hydroxylation to DOPA (1a) and the UV-Vis spectra (A). (i) 100 μM enzyme, 3 mM L-tyrosine and 3 mM H2O2 and three controls with (ii) 100 μM enzyme and 3 mM L-tyrosine, (iii) 3 mM L-tyrosine and 3 mM H2O2 and (iv) 100 μM enzyme and 3 mM H2O2. ITC binding assays showed LmbB2 binds 1 (B), 2 (C) and 3 (D) with KD values of 1.35 ± 0.03, 318 ± 8, and 1294 ± 141 μM, respectively.