Figure 5.

Inhibition of calpain prevents ammonia-induced impairment of synaptic activity. Cultured cerebellar granule cells were treated with ammonia (0, 2, 5, or 10 mM) for 24 h and subjected to patch-clamp electrophysiological recording. Representative tracings (A) and quantification of frequencies and amplitudes (B) of mEPSCs. Representative tracings (C) and quantification of frequencies and amplitudes (D) of mEPSCs in neurons treated with 2 mM ammonia for 24 h with or without ALLM, ZVAD, or AR-A014418. Data are means ± SEs; n =15 cells from three cultures from seven animals; * p < 0.05 vs. Ctrl.