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. 2019 Jul 26;14(7):e0220050. doi: 10.1371/journal.pone.0220050

Fig 2. Human BE refluxates mainly contains conjugated bile acids and stimulates expansion of multi-layered gland at the SCJ in mice.

Fig 2

[a] HPLC analysis of the bile composition of the refluxates of 16 BE patients. [b] Concentration of bile acids in BE patient’s refluxates determined by HPLC. [c,d,e,f,g and h] Analyses of mice after 8 weeks of treatment with human refluxates via oral gavage combined with acidified drinking water pH2.0 (n = 50), acidified drinking water alone (n = 21), or normal tap water (n = 12). [c] Glands (encircled) at the SCJ (line) in mice after treatment. [d] Single staining for K14 and K19 using IHC and double staining for K14 (red) and K19 (green) using IF of MLGS at the SCJ in mice. DAPI (blue) was used as a nuclear counterstain. [e] Percentage of mice showing glands at their SCJ after treatment. [f] Average number of glands at the SCJ per mouse after treatment. Data are represented as (±SD), Unpaired t test *p<0.05, *p<0.01, ***p<0.001, ****p<0.0001. [g] Percentage of mice showing glands at the SCJ, which secrete acidic mucins after treatment. [h] Secretion of acidic mucins in the glandular structures per mouse after treatment, represented by the expression level of Alcian blue staining (S2C Fig). Data are represented as (±SD), Unpaired t test *p<0.05, *p<0.01, ***p<0.001, ****p<0.0001. (Scale bars 100μm).