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. 2019 Jul 26;14(7):e0220050. doi: 10.1371/journal.pone.0220050

Fig 3. Glycine-conjugated BAs are most effective in inducing the development of multi-layered glandular structures and an intestinal type of metaplasia in mice.

Fig 3

[a] H&E and Alcian blue staining of multi-layered glands (MLGS) at the SCJ of mice after 16 weeks of treatment with individual bile components (n = 6 per bile component). [b] Quantification of number of glands and [c] expression level of Alcian blue staining inside the glandular structures (S2C Fig) at the SCJ in mice treated with single BAs for 16 weeks. Treatment with single BAs was compared to treatment with acidified water (pH 2.0). Data are represented as mean±SD. Unpaired t test *p<0.05, *p<0.01, ***p<0.001, ***p<0.0001. Taurocholic (TC), Taurodeoxycholic (TDC), Taurochenodeoxycholic (TCDC), Glycocholic (GC), Glyco-deoxycholic (GDC) and Glycochenodeoxycholic acid (GCDC). [d] Alcian blue staining of MLGS at the SCJ in mice. Goblet cell like structures (encircled) after 16 weeks of glycine-conjugated bile acid treatment. [e] Mucin2 (MUC2) staining of MLGS at the SCJ in mice after treatment with glycine-conjugated bile acids. (Scale bars 100μm).