Table 1. List of all strains used in this study.
| Sample | Site of origin | Spore killer* | Sequenced | Technology | Mycelium | Spok genes† | Spok block location‡ | Flanking genes§ |
|---|---|---|---|---|---|---|---|---|
| Natural isolates¶ | ||||||||
| Wa21– | Wageningen | Psk-2 (Psk-3) | DNA | PacBio | Monokaryon | Spok2, Spok3 | 5R: 3325285 | Pa_5_7950 – Pa_5_7960 |
| HiSeq 2500 | ||||||||
| Wa28– | Wageningen | Psk-2 | DNA | PacBio | Monokaryon | Spok2, Spok3 | 5R: 3325285 | Pa_5_7950 – Pa_5_7960 |
| HiSeq 2500 | ||||||||
| Wa46+ | Wageningen | Naïve (Psk-4) | DNA | PacBio | Monokaryon | SpokΨ1 | – | – |
| HiSeq 2500 | ||||||||
| Wa53– | Wageningen | Psk-1 | DNA | PacBio | Monokaryon | Spok2, Spok3, Spok4 | 3L: 358693 | Pa_3_945 – Pa_3_950 |
| HiSeq 2500 | ||||||||
| Wa58– | Wageningen | Psk-7 | DNA | PacBio | Monokaryon | Spok2, Spok3, Spok4 | 5L: 896822 | Pa_5_490 – Pa_5_470 |
| HiSeq 2500 | ||||||||
| Wa63+ | Wageningen | Psk-S | DNA | PacBio | Monokaryon | Spok2 | – | – |
| HiSeq 2500 | ||||||||
| Wa63– | Wageningen | Psk-S | RNA | HiSeq 2500 | Monokaryon | Spok2 | – | – |
| Wa87+ | Wageningen | Psk-1 | DNA | PacBio | Monokaryon | Spok2, Spok3, Spok4, SpokΨ1 | 3L: 358693 | Pa_3_945 – Pa_3_950 |
| HiSeq 2500 | ||||||||
| Y+ | France | Psk-5 | DNA | MinION | Monokaryon | Spok3, Spok4 | 3L: 358693 | Pa_3_945 – Pa_3_950 |
| HiSeq 2500 | ||||||||
| Wa100+ | Wageningen | Psk-8 | DNA | PacBio | Monokaryon | Spok2, Spok4, SpokΨ1 | 5L: 896822 | Pa_5_490 – Pa_5_470 |
| HiSeq 2500 | ||||||||
| TG+ | France | Psk-5 (sk-1) | DNA | MinION | Monokaryon | Spok3, Spok3, Spok4 | 3L: 358693 | Pa_3_945 – Pa_3_950 |
| DNA | HiSeq X | |||||||
| CBS237.71– | Israel | Psk-P1 | DNA | MinION | Monokaryon | Spok2, Spok3 | 4R: 1674812 | Pa_4_3420 – Pa_4_3410 |
| DNA | HiSeq X | |||||||
| TD+ | ? | Psk-C1 | DNA | HiSeq X | Monokaryon | Spok1 | – | – |
| RNA | HiSeq 2500 | |||||||
| S+ | France | Psk-S | DNA | HiSeq X | Monokaryon | Spok2 | – | – |
| S– | France | Psk-S | DNA | HiSeq X | Monokaryon | Spok2 | – | – |
| Wa47 | Wageningen | naïve (Psk-6) | – | – | – | Not sequenced | – | – |
| Z | France | Psk-7 | – | – | – | Not sequenced | – | – |
| s | France | Psk-S | – | – | – | Not sequenced | – | – |
| Us5 | Germany | Psk-S | – | – | – | Not sequenced | – | – |
| Backcrosses to S†† | ||||||||
| Psk1xS5- (Wa53) | Psk-1 | DNA | HiSeq 2500 | Monokaryon | Spok2, Spok3, Spok4 | 3L: 358693 | Pa_3_945 – Pa_3_950 | |
| Psk2xS5+ (Wa28) | Psk-2 | DNA | HiSeq 2500 | Monokaryon | Spok2, Spok3 | 5R: 3325285 | Pa_5_7950 – Pa_5_7960 | |
| Psk5xS5+ (Y) | Psk-1 (Psk-5) | DNA | HiSeq 2500 | Monokaryon | Spok2, Spok3, Spok4 | 3L: 358693 | Pa_3_945 – Pa_3_950 | |
| Psk7xS5+ (Wa58) | Psk-7 | DNA | HiSeq 2500 | Monokaryon | Spok2, Spok3, Spok4 | 5L: 896822 | Pa_5_490 – Pa_5_470 | |
| Psk1xS14-vsS | Psk-1 | RNA | HiSeq 2500 | Selfing dikaryon | Spok2, Spok3, Spok4 | Like parental | Like parental | |
| Psk2xS14-vsS | Psk-2 | RNA | HiSeq 2500 | Selfing dikaryon | Spok2, Spok3 | Like parental | Like parental | |
| Psk5xS14-vsS | Psk-1 | RNA | HiSeq 2500 | Selfing dikaryon | Spok2, Spok3, Spok4 | Like parental | Like parental | |
| Psk7xS14-vsS | Psk-7 | RNA | HiSeq 2500 | Selfing dikaryon | Spok2, Spok3,+ Spok4 | Like parental | Like parental |
*The spore killer type of each strain is given as reported by van der Gaag et al. (2000) when our phenotyping agrees, and in parenthesis when it does not. The S14 strains were phenotyped by us.
†The S14 Spoks were inferred from RNAseq mapping.
‡The chromosome number and the arm (R for right, and L for left) describing the position of the Spok block are given, along with the coordinates in the Podan2 chromosome.
§ As the exact insertion point is always intergenic, we also provide the flanking genes. The gene nomenclature follows Espagne et al. (2008), where Pa stands for Podospora anserina, the number between underscores is the chromosome and the last number is the gene code.
Note that strain s and strain S are different natural isolates.
††Parentheses denote parental spore killer strains.
‘Like parental’ denotes that the location of the Spok block in the S14 backcrosses was not inferred from sequencing data, however it should correspond to the location in the S5 backcrosses.
'–', Not applicable.