Table 2. Performance of six commercial tests for detecting NS1 antigen and anti-dengue IgM and IgG antibodies.
| Tests | TP | FP | FN | TN | Sensitivity % (95% CI) | Specificity % (95% CI) | PPV % (95% CI) | NPV % (95% CI) | ||
|---|---|---|---|---|---|---|---|---|---|---|
| NS1 | ELISA | InBios | 14 | 1 | 0 | 123 | 100.0 (76.8–100.0) | 99.2 (95.6–99.9) | 93.3 (66.6–98.9) | 100.0 (97.7–100.0) |
| Euroimmun | 14 | 1 | 0 | 123 | 100.0 (76.8–100.0) | 99.2 (95.6–99.9) | 93.3 (66.6–98.9) | 100.0 (97.7–100.0) | ||
| RDT | Asan | 6 | 1 | 8 | 123 | 42.9 (17.7–71.1) | 99.2 (95.6–99.9) | 85.7 (43.7–97.9) | 93.9 (90.7–96.0) | |
| SD | 8 | 0 | 6 | 124 | 57.1 (28.9–82.3) | 100.0 (97.1–100.0) | 100.0 (63.6–100.0) | 95.4 (91.9–97.4) | ||
| Boditech Med | 9 | 0 | 5 | 124 | 64.3 (35.1–87.2) | 100.0 (97.1–100.0) | 100.0 (67.2–100.0) | 96.1 (92.5–98.0) | ||
| IgM | ELISA | InBios | 57 | 2 | 6 | 73 | 90.5 (80.4–96.4) | 97.3 (90.7–99.7) | 96.6 (87.9–99.1) | 92.4 (85.0–96.3) |
| Abcam | 51 | 1 | 12 | 74 | 81.0 (69.1–89.8) | 98.7 (92.8–99.9) | 98.1 (87.9–99.7) | 86.1 (78.7–91.1) | ||
| Euroimmun | 24 | 0 | 39 | 75 | 38.1 (26.1–51.2) | 100.0 (95.2–100.0) | 100.0 (84.1–100.0) | 65.8 (61.3–70.0) | ||
| RDT | Asan | 26 | 0 | 37 | 75 | 41.3 (29.0–54.4) | 100.0 (95.2–100.0) | 100.0 (85.2–100.0) | 66.9 (62.2–71.4) | |
| SD | 31 | 1 | 32 | 74 | 49.2 (36.4–62.1) | 98.7 (92.8–99.9) | 96.9 (81.3–99.5) | 69.8 (64.4–74.7) | ||
| Boditech Med | 54 | 6 | 9 | 69 | 85.7 (74.6–93.3) | 92.0 (83.4–97.0) | 90.0 (80.6–95.1) | 88.5 (80.7–93.4) | ||
| IgG | ELISA | InBios | 68 | 1 | 2 | 67 | 97.1 (90.1–99.7) | 97.7 (87.9–99.9) | 98.6 (90.7–99.8) | 97.1 (89.5–99.2) |
| Abcam | 70 | 7 | 0 | 61 | 100.0 (94.9–100.0) | 89.7 (79.9–95.8) | 90.9 (83.2–95.3) | 100.0 (94.1–100.0) | ||
| Euroimmun | 66 | 1 | 4 | 67 | 94.3 (86.0–98.4) | 98.5 (92.1–99.9) | 98.5 (90.4–99.8) | 94.4 (86.6–97.7) | ||
| RDT | Asan | 46 | 0 | 24 | 68 | 65.7 (53.4–76.6) | 100.0 (94.7–100.0) | 100.0 (91.5–100.0) | 73.9 (67.2–79.7) | |
| SD | 50 | 0 | 20 | 68 | 71.4 (59.4–81.6) | 100.0 (94.7–100.0) | 100.0 (92.2–100.0) | 77.3 (70.1–83.1) | ||
| Boditech Med | 66 | 1 | 4 | 67 | 94.3 (86.0–98.4) | 98.5 (92.1–99.9) | 98.5 (90.4–99.8) | 94.3 (86.6–97.7) | ||
| NS1+IgM | ELISA | InBios | 65 | 2 | 3 | 68 | 95.6 (87.6–99.1) | 97.1 (90.1–99.6) | 97.0 (89.2–99.2) | 95.8 (88.2–98.6) |
| Euroimmun | 32 | 0 | 36 | 70 | 47.1 (34.8–59.6) | 100 (94.9–100.0) | 100.0 (87.8–100.0) | 66.0 (60.9–70.9) | ||
| RDT | Asan | 30 | 1 | 38 | 69 | 44.1 (32.1–56.7) | 98.6 (92.3–99.9) | 96.8 (80.8–99.5) | 64.5 (59.5–69.2) | |
| SD | 35 | 1 | 33 | 69 | 51.5 (39.0–63.8) | 98.6 (92.3–99.9) | 97.2 (83.1–99.6) | 67.6 (62.0–72.8) | ||
| Boditech Med | 60 | 5 | 8 | 65 | 88.2 (78.1–94.8) | 92.9 (84.1–97.6) | 92.3 (83.7–96.6) | 89.0 (80.9–93.9) | ||
| NS1+IgG+ IgM | ELISA | InBios | 75 | 3 | 0 | 60 | 100.0 (95.2–100.0) | 95.2 (86.7–99.0) | 96.2 (89.2–98.7) | 100.0 (94.5–100.0) |
| Abcam | 71 | 7 | 4 | 56 | 94.7 (86.9–98.5) | 88.9 (78.4–95.4) | 91.0 (83.4–95.3) | 93.3 (84.3–97.3) | ||
| Euroimmun | 74 | 1 | 1 | 62 | 98.7 (92.8–99.9) | 98.4 (91.5–99.9) | 98.7 (91.4–99.8) | 93.4 (89.8–99.8) | ||
| RDT | Asan | 58 | 1 | 17 | 62 | 77.3 (66.3–86.2) | 98.4 (91.5–99.9) | 98.3 (89.2–99.8) | 78.5 (70.6–84.7) | |
| SD | 62 | 0 | 13 | 63 | 82.7 (72.2–90.4) | 100.0 (94.3–100.0) | 100.0 (93.9–100.0) | 82.9 (74.7–88.8) | ||
| Boditech Med | 74 | 6 | 1 | 57 | 98.7 (92.8–99.9) | 90.5 (80.4–96.4) | 92.5 (85.2–96.4) | 98.3 (89.0–99.8) | ||
Abbreviations: SD, Standard Diagnostics; NS1, non-structural-1; CI, confidence interval; RDT, rapid diagnostic test; TP, true positive; FN, false negative; TN, true negative; FP, False positive; PPV, positive predictive value; NPV, negative predictive value.