Fig. 3.

Inhibition effect of compounds on C. trachomatis L2. HeLa cells were infected with C. trachomatis L2 EBs at a MOI of 0.2 in the presence of 0.5% DMSO, 11.25 μM tetracycline (Tet) or various concentrations of compounds 1–3. (A) Cells were fixed 36 hours post-infection. The chlamydial inclusions were strained with a polyclonal anti-EB antibody (green) and cells were counterstained with Evans blue (red). (B) The inhibitory effect of compounds on the production of infectious progeny EBs was determined by counting recoverable IFUs in compound or Tet treated samples relative to untreated negative control. Asterisks indicate compound 1 inhibition levels were significantly different from compound 2 and 3 (*P<0.05, **P<0.01 by Student’s t test). (Color figure available online only)