Noct WT mice were treated with either Sacch or Dox beginning at 4 weeks of age and followed till 16 weeks of age. A) Areal Bone mineral density (aBMD) was measured by DXA at 8, 12 and 16 weeks (n=6–12). B) Representative μCT images of trabecular bone (Tb); followed by femoral microarchitecture trabecular parameters measured at 16 weeks. Tb. BV/TV, Tb.Th, Tb.N, Tb.Sp and Conn.D. C) Representative μCT images of cortical bone (Ct.) and femoral microarchitecture cortical parameters measured Ct.Ar/Tt.Ar, Ct.Ar, Tt.Ar, Ma.Ar, Ct.Porosity, Ct.Th and Ct.TMD are shown. Values represent mean ± SEM of n=4–5. p values are calculated as described in methods section for A and using an unpaired students t-test in GraphPad Prism for B,C.