Figure 3. Immunogenicity of Ni-NTA-AuNP-supported MPER-TMD proteoliposomes.
(a) Immunization schedule of 5 guinea pigs. Serum samples were collected at weeks 4 and 12. (b) ELISA analysis of serum for anti-MPER-TMD antibodies. ELISA plates were coated with MPER-TMD in regular liposomes. Sera were added in serial dilutions (2700, 900, 300, 100) and detected with a horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig secondary antibody for total IgG ELISAs. (c) ELISA plate incubated with empty liposomes.